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anti phospho rps6 antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti phospho rps6 antibody
    Anti Phospho Rps6 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rps6/Phospho-S6+Ribosomal+Protein+(Ser240%2F244)+XP+Rabbit+mAb/bio_rxiv__64898__2026__03__27__714790-190-9-13
    Average 96 stars, based on 1113 article reviews
    anti phospho rps6 antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Characterizing molecular and behavioral changes arising from ROMK potassium channel deficiency in the cerebellum
    Article Snippet: .. For immunofluorescence, the primary antibodies used were anti-calbindin (1:1000, Proteintech, Cat. No. 66394-1-Ig, Host: Mouse), anti-calbindin (1:1000, Proteintech, Cat. No. 14479-1-AP, Host: Rabbit), anti-GFAP (1:250, Cell Signaling Technology, Cat. No. 3670, Host: Mouse) and anti-rpS6 (1:500, Cell Signaling Technology, Cat. No. 2217, Host: Rabbit). ..

    other:

    Article Title: Gene expression dynamics before and after zygotic gene activation in Drosophila early embryogenesis
    Article Snippet: anti-RpS6 , Cell Signaling Technology , #2317; RRID: AB_2238583.

    Article Title: Vitamin D Regulates Olfactory Function via Dual Transcriptional and mTOR‐Dependent Translational Control of Synaptic Proteins
    Article Snippet: The following antibodies were used (if not mentioned in the immunohistochemistry section): mouse anti‐β‐actin (1:200, Novus, NB600‐501), rabbit anti‐AKT (1:200, Cell Signaling Technology, 4691), rabbit anti‐p‐AKT (1:20, Cell Signaling Technology, 4060), rabbit anti‐eIF4E (1:100, Cell Signaling Technology, 2067), rabbit anti‐eIF4EBP1 (1:100, Cell Signaling Technology, 9644), rabbit anti‐p‐eIF4EBP1 (1:200, Cell Signaling Technology, 2855), rabbit anti‐eIF4EBP2 (1:20, CST, 2845), rabbit anti‐GAPDH (1:5000, Cell Signaling Technology, 5174), mouse anti‐gephyrin (1:4000, Synaptic Systems, 147 111), rabbit anti‐GFP (1:1000; Invitrogen A‐11122), rabbit anti‐mGluR1 (1:500, Cell Signaling Technology, 12 551), rabbit anti‐MNK (1:100, Cell Signaling Technology, 2195), rabbit anti‐p‐MNK (1:20, Cell Signaling Technology, 2111), mouse anti‐mTOR (1:1000, CST, 4517), rabbit anti‐p‐mTOR (1:50, CST, 5536), rabbit anti‐PI3K (1:3000, Cell Signaling Technology, 4249), rabbit anti‐p‐PI3K (1:20, Cell Signaling Technology, 17 366), rabbit anti‐PSD95 (1:100, Cell Signaling Technology, 3409), rabbit anti‐Rps6 (1:100, Cell Signaling Technology, 2217), rabbit anti‐S6K (1:200, CST, 33 475), rabbit anti‐p‐S6K (1:50, CST, 9234), mouse anti‐synapsin1 (1:7000, Synaptic Systems, 106 011), mouse anti‐VGAT (1:20, Synaptic Systems, 131 011), mouse anti‐vGlut1 (1:7000, Synaptic Systems, 135 011), and rabbit anti‐vGlut2 (1:100, Cell Signaling Technology, 16 066).

    Control:

    Article Title: Nitric Oxide-Mediated S-Nitrosylation of TSC2 Drives mTOR dysregulation across Shank3 and Cntnap2 Models of Autism Spectrum Disorder.
    Article Snippet: .. Primary antibodies, anti-p-mTOR (#5536), anti-mTOR (#2983), antip-RPS6 (#4858), anti-RPS6 (#2317S), anti-β-actin (#3700), AntiNeuN (#94403), anti-TSC2 (#4308), IgG Isotype control (3900S), CaMKII-alpha (50049S) and secondary antibodies, Anti-Rabbit Alexa fluor 594 (#8889), Anti-mouse Alexa Fluor 488 (#4408), HRP-conjugated anti-rabbit (7076S), HRP conjugated anti-mouse (7074S), ProLong gold Antifade with DAPI (#8961), and protease phosphatase inhibitor cocktail (#5872) were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Anti-p70 S6 kinase (ab2571), anti-MAP2 (ab11268), anti-MAP2 (AB5392), Goat AntiChicken Alexa Fluor 647 (AB-ab150175) were purchased from Abcam, Cambridge, UK.

    SDS Page:

    Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs
    Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), anti-Rps6 (1:5000, rabbit, Cell Signaling 2217), anti-Fau (1:500, Proteintech 13,581-1-AP), anti-Rplp2 (1:1000, Invitrogen PA527541), anti-Rps26 (1:500, Proteintech 14,909-1-AP), anti-Rpl3 (1:2000, Proteintech 11,005-1-AP), anti-Rpl3l (1:500, Antibodies Online ABIN2706985), anti-Rpl36 (1:500, Proteintech 15,145-1-AP), anti-H3 (1:10 000, Cell Signaling 9715), anti-HSP60 (1:2000, Santa Cruz sc-376240), anti-GAPDH (1:5000, Proteintech 60,004-1-Ig). .. On the following day, membranes were washed and then incubated with horseradish peroxidase-linked secondary antibodies: anti-rabbit (Proteintech SA00001-2) or anti-mouse (Millipore 12–349) diluted to 1:5000 or 1:10 000 in TBS-T. Membranes were probed with ECL substrate (100 mM Tris HCl pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich) and 0.15% H 2 O 2 (Sigma-Aldrich)).

    Electrophoresis:

    Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs
    Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), anti-Rps6 (1:5000, rabbit, Cell Signaling 2217), anti-Fau (1:500, Proteintech 13,581-1-AP), anti-Rplp2 (1:1000, Invitrogen PA527541), anti-Rps26 (1:500, Proteintech 14,909-1-AP), anti-Rpl3 (1:2000, Proteintech 11,005-1-AP), anti-Rpl3l (1:500, Antibodies Online ABIN2706985), anti-Rpl36 (1:500, Proteintech 15,145-1-AP), anti-H3 (1:10 000, Cell Signaling 9715), anti-HSP60 (1:2000, Santa Cruz sc-376240), anti-GAPDH (1:5000, Proteintech 60,004-1-Ig). .. On the following day, membranes were washed and then incubated with horseradish peroxidase-linked secondary antibodies: anti-rabbit (Proteintech SA00001-2) or anti-mouse (Millipore 12–349) diluted to 1:5000 or 1:10 000 in TBS-T. Membranes were probed with ECL substrate (100 mM Tris HCl pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich) and 0.15% H 2 O 2 (Sigma-Aldrich)).

    Membrane:

    Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs
    Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), anti-Rps6 (1:5000, rabbit, Cell Signaling 2217), anti-Fau (1:500, Proteintech 13,581-1-AP), anti-Rplp2 (1:1000, Invitrogen PA527541), anti-Rps26 (1:500, Proteintech 14,909-1-AP), anti-Rpl3 (1:2000, Proteintech 11,005-1-AP), anti-Rpl3l (1:500, Antibodies Online ABIN2706985), anti-Rpl36 (1:500, Proteintech 15,145-1-AP), anti-H3 (1:10 000, Cell Signaling 9715), anti-HSP60 (1:2000, Santa Cruz sc-376240), anti-GAPDH (1:5000, Proteintech 60,004-1-Ig). .. On the following day, membranes were washed and then incubated with horseradish peroxidase-linked secondary antibodies: anti-rabbit (Proteintech SA00001-2) or anti-mouse (Millipore 12–349) diluted to 1:5000 or 1:10 000 in TBS-T. Membranes were probed with ECL substrate (100 mM Tris HCl pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich) and 0.15% H 2 O 2 (Sigma-Aldrich)).

    Saline:

    Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs
    Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), anti-Rps6 (1:5000, rabbit, Cell Signaling 2217), anti-Fau (1:500, Proteintech 13,581-1-AP), anti-Rplp2 (1:1000, Invitrogen PA527541), anti-Rps26 (1:500, Proteintech 14,909-1-AP), anti-Rpl3 (1:2000, Proteintech 11,005-1-AP), anti-Rpl3l (1:500, Antibodies Online ABIN2706985), anti-Rpl36 (1:500, Proteintech 15,145-1-AP), anti-H3 (1:10 000, Cell Signaling 9715), anti-HSP60 (1:2000, Santa Cruz sc-376240), anti-GAPDH (1:5000, Proteintech 60,004-1-Ig). .. On the following day, membranes were washed and then incubated with horseradish peroxidase-linked secondary antibodies: anti-rabbit (Proteintech SA00001-2) or anti-mouse (Millipore 12–349) diluted to 1:5000 or 1:10 000 in TBS-T. Membranes were probed with ECL substrate (100 mM Tris HCl pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich) and 0.15% H 2 O 2 (Sigma-Aldrich)).

    Incubation:

    Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs
    Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), anti-Rps6 (1:5000, rabbit, Cell Signaling 2217), anti-Fau (1:500, Proteintech 13,581-1-AP), anti-Rplp2 (1:1000, Invitrogen PA527541), anti-Rps26 (1:500, Proteintech 14,909-1-AP), anti-Rpl3 (1:2000, Proteintech 11,005-1-AP), anti-Rpl3l (1:500, Antibodies Online ABIN2706985), anti-Rpl36 (1:500, Proteintech 15,145-1-AP), anti-H3 (1:10 000, Cell Signaling 9715), anti-HSP60 (1:2000, Santa Cruz sc-376240), anti-GAPDH (1:5000, Proteintech 60,004-1-Ig). .. On the following day, membranes were washed and then incubated with horseradish peroxidase-linked secondary antibodies: anti-rabbit (Proteintech SA00001-2) or anti-mouse (Millipore 12–349) diluted to 1:5000 or 1:10 000 in TBS-T. Membranes were probed with ECL substrate (100 mM Tris HCl pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich) and 0.15% H 2 O 2 (Sigma-Aldrich)).

    Western Blot:

    Article Title: Gene expression dynamics before and after zygotic gene activation in Drosophila early embryogenesis
    Article Snippet: Protein bands were detected using the Super Signal West Pico chemiluminescent substrate (Thermo Fisher Scientific Inc., Waltham, MA, USA) on a ChemiDoc Imaging System. .. Primary antibodies used for western blotting were: Anti-Ago1 (ab5070; Abcam, Cambridge, UK), anti-HSP83 (anti-HSP90 antibody, #4874; Cell Signaling Technology, Danvers, MA, USA), anti-Wisp, anti-RpS6 (#2317; Cell Signaling Technology), anti-Me31B, and anti-PABP. .. Embryo samples were lysed in lysis buffer (8 M urea, 50 mM ammonium bicarbonate, 1× protease inhibitor cocktail (Calbiochem, San Diego, CA, USA), and RNasin RNase inhibitor (Promega, Madison, WI, USA) and first treated with 10 mM DTT (Sigma-Aldrich, St. Louis, MO, USA).



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    Cell Signaling Technology Inc rps6
    (A) Western blot analysis of total <t>Rps6</t> and phospho-Rps6 levels in rpl12aΔ and rpl12bΔ strains relative to wild type (WT). Histone H3 was used as a loading control. (B ) Quantification of total Rps6 and phospho-Rps6 protein levels normalized to WT. (C) Phospho-Rps6 occupancy analysis comparing rpl12bΔ and rpl12aΔ across transcripts grouped by coding sequence (CDS) length bins. (D, E) Yeast two-hybrid (Y2H) assays were performed to assess potential direct interactions between TOR regulators and Rpl12 paralogs. Error bars represent SEM.
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    Image Search Results


    (A) Western blot analysis of total Rps6 and phospho-Rps6 levels in rpl12aΔ and rpl12bΔ strains relative to wild type (WT). Histone H3 was used as a loading control. (B ) Quantification of total Rps6 and phospho-Rps6 protein levels normalized to WT. (C) Phospho-Rps6 occupancy analysis comparing rpl12bΔ and rpl12aΔ across transcripts grouped by coding sequence (CDS) length bins. (D, E) Yeast two-hybrid (Y2H) assays were performed to assess potential direct interactions between TOR regulators and Rpl12 paralogs. Error bars represent SEM.

    Journal: bioRxiv

    Article Title: Rpl12 paralog dependent TOR-signaling controls the expression of ribosome preservation factor Stm1

    doi: 10.64898/2026.03.25.714144

    Figure Lengend Snippet: (A) Western blot analysis of total Rps6 and phospho-Rps6 levels in rpl12aΔ and rpl12bΔ strains relative to wild type (WT). Histone H3 was used as a loading control. (B ) Quantification of total Rps6 and phospho-Rps6 protein levels normalized to WT. (C) Phospho-Rps6 occupancy analysis comparing rpl12bΔ and rpl12aΔ across transcripts grouped by coding sequence (CDS) length bins. (D, E) Yeast two-hybrid (Y2H) assays were performed to assess potential direct interactions between TOR regulators and Rpl12 paralogs. Error bars represent SEM.

    Article Snippet: Membranes were blocked with 5% BSA for 1 hour at room temperature incubated overnight at 4 °C with primary antibodies (1:2000 dilution) against Rpl12 (Invitrogen), phospho-Rps6 (Ser235/236), Rps6, and Histone H3 (Cell Signaling).

    Techniques: Western Blot, Control, Sequencing