anti phospho rps6 antibody (Cell Signaling Technology Inc)
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Anti Phospho Rps6 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rps6/Phospho-S6+Ribosomal+Protein+(Ser240%2F244)+XP+Rabbit+mAb/bio_rxiv__64898__2026__03__27__714790-190-9-13
Average 96 stars, based on 1113 article reviews
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Immunofluorescence:Article Title: Characterizing molecular and behavioral changes arising from ROMK potassium channel deficiency in the cerebellum Article Snippet: .. For immunofluorescence, the primary antibodies used were anti-calbindin (1:1000, Proteintech, Cat. No. 66394-1-Ig, Host: Mouse), anti-calbindin (1:1000, Proteintech, Cat. No. 14479-1-AP, Host: Rabbit), anti-GFAP (1:250, Cell Signaling Technology, Cat. No. 3670, Host: Mouse) and other:Article Title: Gene expression dynamics before and after zygotic gene activation in Drosophila early embryogenesis Article Snippet: Article Title: Vitamin D Regulates Olfactory Function via Dual Transcriptional and mTOR‐Dependent Translational Control of Synaptic Proteins Article Snippet: The following antibodies were used (if not mentioned in the immunohistochemistry section): mouse anti‐β‐actin (1:200, Novus, NB600‐501), rabbit anti‐AKT (1:200, Cell Signaling Technology, 4691), rabbit anti‐p‐AKT (1:20, Cell Signaling Technology, 4060), rabbit anti‐eIF4E (1:100, Cell Signaling Technology, 2067), rabbit anti‐eIF4EBP1 (1:100, Cell Signaling Technology, 9644), rabbit anti‐p‐eIF4EBP1 (1:200, Cell Signaling Technology, 2855), rabbit Control:Article Title: Nitric Oxide-Mediated S-Nitrosylation of TSC2 Drives mTOR dysregulation across Shank3 and Cntnap2 Models of Autism Spectrum Disorder. Article Snippet: .. Primary antibodies, anti-p-mTOR (#5536), anti-mTOR (#2983), antip-RPS6 (#4858), SDS Page:Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), Electrophoresis:Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), Membrane:Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), Saline:Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), Incubation:Article Title: Proteomics-based characterization of ribosome heterogeneity in adult mouse organs Article Snippet: After denaturation at 95 °C for 5 min, the protein concentration of all fractions was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific). .. 20 μg of protein were loaded on a 12% SDS-PAGE gel and separated by electrophoresis for 4 h at 230 V. Proteins were then transferred on nitrocellulose membrane (Thermo Scientific) under a constant amperage of 250 mA for 3 h. Membrane was blocked in fat free 5% milk powder in Tris-Buffered Saline (TBS) and incubated with the following primary antibodies diluted in 5% fat free milk powder in TBS with 0.1% tween (TBS-T) overnight under agitation at 4 °C: anti-Rpl22 (1:2000, mouse, Santa Cruz sc-373993), Western Blot:Article Title: Gene expression dynamics before and after zygotic gene activation in Drosophila early embryogenesis Article Snippet: Protein bands were detected using the Super Signal West Pico chemiluminescent substrate (Thermo Fisher Scientific Inc., Waltham, MA, USA) on a ChemiDoc Imaging System. .. Primary antibodies used for western blotting were: Anti-Ago1 (ab5070; Abcam, Cambridge, UK), anti-HSP83 (anti-HSP90 antibody, #4874; Cell Signaling Technology, Danvers, MA, USA), anti-Wisp, |
